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KMID : 1189420070200020047
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2007 Volume.20 No. 2 p.47 ~ p.67
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The effect of Daehwanggogasangbakpi(ÓÞüÜÍÇÊ¥ßÍÛÜù«) on the skin damage induced by ultraviolet irradiation
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Lee Hyun-Woo
Hong Seung-Ug
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Abstract
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Objective: As a result of increasing amount of ultraviolet ray, skin problems including sunburn, rapid skin aging, melanoma, and even skin cancer continue to rise. In the present study, the effect of oriental herbal extract, Daehwanggo(ÓÞüÜÍÇ,DH) and Daehwanggogasnagbakpi(ÓÞüÜÍÇÊ¥ßÍÛÜù«,DS), as external application, on the skin damage, was investigated.
Methods: 30 mice were equally distributed into 3 groups : control, UVB-control and UVB-irradiated and DS-treated group. Also mouse melanoma cell lines were cultured. Tyrosinase inhibition was measured to analyze the UN-protection effect. Melanogenesis in the UV-irradiated melanoma cell lines was compared in DS-treated cell line and control cell line. Sample skin from the ear tissue of the 3 groups were analyzed to observe the inflammatory response, T cell differentiation, apoptosis of keratinocytes.
Results: The tyrosinase was more significantly inhibited in the DS group compared to DH group. Antioxidative effects was more prominent in DS group when superoxide dismutase was measured. Both the DS- and DH-treated cell lines showed significantly reduced melanogenesis. The reduction of external skin damage including erythematous papule, eczema, keratinocyte, pyopoiesis was observed in the DS- and DH-treated sample cells. In terms of the effect on the skin damage, sunburn cell, activated skin mast cells, secretion of IL-12, manifestation of HSP70, hyperplasia of epithelial cells, MMP-9 and destruction of the collagen were all significantly improved in the DS-treated sample cells. Melanin cells and the apoptosis in the melanoma cell line were decreased.
Conclusion: DH and DS were traditionally applied externally for the scald in the oriental medicine. The present study elucidated the possibility of herbal extracts to be used as ultraviolet protectives. Further investigations are needed to assure the clinical application.
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KEYWORD
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Daehwanggo(ÓÞüÜÍÇ), Daehwanggogasnagbakpi(ÓÞüÜÍÇÊ¥ßÍÛÜù«), melanogenesis, UVprotection
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